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Keywords: Spike detection; Spike sorting; Spike train analysis; Extra-cellular recording; Tetrode; Electrophysiology; Multi-unit recording; Single-unit recording; Cluster analysis; Superposition resolution; MATLAB; Accuracy quantification; Object Oriented Programming;
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WaterFillThresh Tool                    



Summary

The WaterfillThresh tool is a Fragmentation tool within the Spike Detection toolbox, used to detect spikes based on a specific threshold routine. The “Waterfill” name comes from the following analogy: Picture a model of a mountain range in a tub filled with water so that you cannot see the mountains. As you drain the tub you will be able to see the tallest peak first, then the next tallest peak, and so on as the water level drops.

As each peak is reached, a fragment of data (spike) is saved to disk, based on user-selected criteria.

Screen Shot




Details

The WaterfillThresh spike detection algorithm starts by finding the largest data point in the entire data set. This is a distinct contrast to traditional threshold routines, which search the data file in a sequential fashion.

After finding the high point with the raw data file, WaterfillThresh selects a user-specified number of points before and after this point; this data range becomes the first fragment, or spike. As in the traditional threshold routine, a predetermined number of points before and after the high point are skipped. Skipping a user-selected number of points between spikes removes the risk of creating a second spike around the same high data point.

WaterfillThresh then finds the next largest point and selects data surrounding it as the second spike, and so on until a user-defined minimum threshold is met. An automatic 3 Sigma or 2 Sigma threshold is also available.

The two main advantages of WaterfillThresh are:

1. By focusing on the largest points first, you can get the best fragments first (in case you are limiting the number of fragments that you are taking).

2. Because WaterfillThresh focuses on the peaks, the fragments will initially be better aligned, making display and further processing easier.

 
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Last Updated: 25-Mar-2008